치과용 레이저 조사가 발치와 치유과정에 관련된 세포에 미치는 영향에 관한 연구
THE EFFECTS OF DENTAL LASER ON THE SOCKET CELLS, INVOLVING THE EFFECTS OF DENTAL LASER ON THE SOCKET CELLS, INVOLVING HEALING PROCESS AFTER TOOTH EXTRACTION
소속 상세정보
신제원/Je Won Shin
KMID : 0355819990230010013
Abstract
-Abstract-
In order to verify the acceleration effect of Ca-As Semi-Conductor-Laser (Frequency:
5 Hz∼10,000 Hz Model: SD-101a RCA, U.S.A.)) were examined in vitro using rat cells
obtained from the coagulum in sockets 2 days after tooth extration. The mitogenic effect
of soft laser was assessed by the MTT assay. The morphologic changes after soft laser
irradiation were investigated with the scanning and transmission electron microscope.
The results are as follows;
1. The mitogenic effect of the soft laser was gradually increasing until 3 times
irradiation, while the mitogenic response at 4th time was decreasing compared to the
control value.
2. The mitogenic effects of the soft laser on pulp fibroblast were not difference by the
amount of the frequency.
3. In scanning electron microscopic study, The process of cell surface increased
gradually with width and length after laser radiation. It demonstrates that the
development of process is closely associated with the differentiation of cells.
4. Under the transmission electron microscope, The laser-irradiated cells maintained their
elongated shape and a high degree of cellular polarization. The large cell body contains
a well developed Golgi complex, a large number of profiles of rough endoplasmic
reticulum, and great numbers of mitochondria.
These findings suggest that the laser may have important roles in promotion of cell
proliferation and consequently may be useful for clinical application in socket healing
procedures.
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